Effects of Zn and oxytetracycline on mobile genetic elements, antibiotic resistance genes, and microbial community evolution in soil.

Antibiotics and heavy metals added to livestock and poultry feed are excreted in manure, which is added to agricultural soil and causes severe pollution. However, the effects of oxytetracycline (OTC) and zinc (Zn), which are present at relatively high levels in feed additives, on antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), and microbial communities have not been comprehensively studied. This study evaluated the effects of OTC and Zn on environmental factors, microorganisms, MGEs, and ARGs. The expression of MGEs in soil was stimulated by adding Zn at concentrations of 500 and 1000 mg/kg or OTC at concentrations of 30 and 100 mg/kg; however, the addition of their combination hindered the expression of MGEs in soil. The abundance of total MGEs and ARGs tended to decrease with increasing concentrations of Zn and OTC and the number of incubation days. Low and high OTC concentrations strongly inhibited sul and tet resistance genes, respectively. Network analysis showed that changes in the population of Firmicutes and Proteobacteria had the greatest impact on ARG abundance. Redundancy analysis revealed that MGEs, particularly intI2, facilitated the transfer and spread of ARGs and had the greatest impact on changes in ARG abundance. These findings provide reference values for the prevention and resolution of ecological and environmental risks posed by the presence of Zn and OTC in organic manure soil.

In situ synthesis of Fe-N co-doped carbonaceous nanocomposites using biogas residue as an effective persulfate activator for remediation of aged petroleum contaminated soils.

Persulfate (PS)-based chemical oxidation is an effective method for the remediation of petroleum-contaminated soils, but higher concentrations of PS (3-40%) may lead to soil acidification (pH decreased by 1.8-6.2 units) and affect the microbial communities. In this study, Fe/N co-doped carbonaceous nanocomposites (Fe-N @ CN) that can efficiently activate PS were developed from biogas residue for the remediation of petroleum-contaminated soil. The as-obtained Fe-N@CN displayed that the Fe-based nanoparticles were encapsulated in graphitic nanosheets, with Fe3C and FeN0.0760 as the main bonding modes. The removal efficiency of total petroleum hydrocarbons (TPHs) reached 73.14% in 3 days with a PS dose of 2% and catalyst dose of 0.4%, and increased by 15.8% on adding 30 mmol/kg of ß-cyclodextrin. The free-radical quenching experiment and electron paramagnetic resonance revealed that SO4·-,·OH, O2·-, and 1O2 were involved in the removal of TPHs. Because of the low PS dosage, the remediation process had no significant effect on the soil pH. During the remediation process, soil catalase activity was enhanced and then recovered, whereas the soil bacterial community, reflected by the operational taxonomic unit values, decreased and then recovered. TPH-degrading bacteria were produced in the Fe-N@CN/PS/soil system after chemical oxidation, further contributing to soil remediation.